General description

A cell-permeable pyrrazolopyrimidine compound that acts as a potent, selective, reversible, and ATP-competitive inhibitor of AMPK (AMP-activated protein kinase; K_i = 109 nM in the presence of 5 µM ATP and the absence of AMP). Does not affect the activities of ZAPK, Syk, PKCθ, PKA, or JAK3. Blocks celluar activities induced by AICAr (Cat. No. 123040) or Metformin (Cat. No. 317240). Induces weight loss by attenuating AMPK-mediated food intake in mice. The solid form of this compound (Cat. No. 171260) is also available.

A cell-permeable pyrrazolopyrimidine compound that acts as a potent, selective, reversible, and ATP-competitive inhibitor of AMPK (AMP-activated protein kinase; K_i = 109 nM in the presence of 5 µM ATP and the absence of AMP). Does not affect the activities of ZAPK, Syk, PKCθ, PKA, or JAK3. Blocks celluar activities induced by AICAr (Cat. No. 123040) or Metformin (Cat. No. 317240). Induces weight loss by attenuating AMPK-mediated food intake in mice.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Yu, P.B., et al. 2008. Nat. Chem. Biol4, 33.Kim, E.K., et al. 2004. J. Biol. Chem.279, 19970.Lee, M., et al. 2003. J. Biol. Chem.278, 39653.Inoki, K., et al. 2003. Cell115, 577.Fryer, L.G., 2002. FEBS Lett.531, 189.Zhou, G., et al. 2001. J. Clin. Invest.108, 1167.

Packaging

Packaged under inert gas

2 mg in Glass bottle

Physical form

A 10 mM (2 mg/408 µl) solution of AMPK Inhibitor, Compound C, 2HCl, (Cat. No. 171260) in H₂O.

Reconstitution

Following intial thaw, aliquot and freeze (-20°C).